|
|
|
|
|
| Siddique, Mohammad A. Momin; Butts, Ian A. E.; Linhart, Otomar; Macias, Adrian D.; Fauvel, Christian. |
| Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of... |
| Tipo: Text |
Palavras-chave: Egg longevity; Overripe eggs; Reproductive ecology; Mediterranean sea bass; Dicentrarchus labrax. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00279/39000/37580.pdf |
| |
|
|
| Politis, Sebastian Nikitas; Sorensen, Sune R.; Mazurais, David; Servili, Arianna; Zambonino-infante, Jose-luis; Miest, Joanna J.; Clemmesen, Catriona M.; Tomkiewicz, Jonna; Butts, Ian A. E.. |
| Digestive system functionality of fish larvae relies on the onset of genetically pre-programmed and extrinsically influenced digestive functions. This study explored how algal supplementation (green-water) until 14 days post hatch (dph) and the ingestion of food [enriched rotifer (Brachionus plicatilis) paste] from 15 dph onward affects molecular maturation and functionality of European eel larval ingestion and digestion mechanisms. For this, we linked larval biometrics to expression of genes relating to appetite [ghrelin (ghrl), cholecystokinin (cck)], food intake [proopiomelanocortin (pomc)], digestion [trypsin (try), triglyceride lipase (tgl), amylase (amyl)], energy metabolism [ATP synthase F0 subunit 6 (atp6), cytochrome-c-oxidase 1 (cox1)], growth... |
| Tipo: Text |
Palavras-chave: Anguilla anguilla; Ingestion; Digestion; Gene expression; RNA/DNA; Aquaculture. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57445/59550.pdf |
| |
|
|
| Politis, Sebastian Nikitas; Mazurais, David; Servili, Arianna; Zambonino-infante, Jose-luis; Miest, Joanna J.; Tomkiewicz, Jonna; Butts, Ian A. E.. |
| European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2 alpha, nkcc2 beta, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thr alpha A, thr alpha B, thr beta B, diol, dio2, dio3) were differentially expressed... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00448/55982/57456.pdf |
| |
|
|
| Politis, Sebastian Nikitas; Mazurais, David; Servili, Arianna; Zambonino, Jose-luis; Miest, Joanna J.; Sorensen, Sune R.; Tomkiewicz, Jonna; Butts, Ian A. E.. |
| Temperature is important for optimization of rearing conditions in aquaculture, especially during the critical early life history stages of fish. Here, we experimentally investigated the impact of temperature (16, 18, 20, 22 and 24°C) on thermally induced phenotypic variability, from larval hatch to first-feeding, and the linked expression of targeted genes [heat shock proteins (hsp), growth hormone (gh) and insulin-like growth factors (igf)] associated to larval performance of European eel, Anguilla anguilla. Temperature effects on larval morphology and gene expression were investigated throughout early larval development (in real time from 0 to 18 days post hatch) and at specific developmental stages (hatch, jaw/teeth formation, and first-feeding).... |
| Tipo: Text |
|
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50616/51328.pdf |
| |
|
|
|
